Analysis of Acetylcholinesterase Activity in Cell Membrane Microarrays of Brain Areas as a Screening Tool to Identify Tissue Specific Inhibitors

Acetylcholinesterase (AChE) is responsible for hydrolyzing the acetylcholine neurotransmitter, bringing an end point to cholinergic neurotransmission. Thus, AChE primary target of a wide spectrum compounds used as pesticides, nerve agents or therapeutic drugs neurodegenerative diseases such Alzheimer’s disease (AD). This enzyme heterogeneously distributed in brain showing different activity depending on nervous region. Therefore, aim this work report novel technology that enables simultaneous determination tissue specific activity, well analysis and screening inhibitors, by using cell membrane microarrays. These microarrays were composed membranes, isolated from 41 tissues, organs areas, immobilized over slide, maintaining functionality proteins. To validate platform, demonstrating its usefulness drug discovery high throughput tool, colorimetric protocol detect membrane-bound was optimized. rat cortical striatal activities estimated presence increased concentrations donepezil effect assessed simultaneously tissues organs, major potential microarray’s technology.